![]() Taurine, 2‐aminoethane sulfonic acid, is involved in several biological processes for example, serving as a precursor for the tauro‐conjugated bile salts, for example, taurocholate for cellular volume regulation (for references see the excellent review by Huxtable ( 1992)) and being important for retinal homeostasis through its function as an antioxidant and osmolyte (Huxtable 1992) (Ripps and Shen 2012). ![]() In vivo and in vitro results suggest that taurine absorption from the intestine is caused by PAT1. In vitro it was clearly shown that PAT1 mediated the cellular uptake of taurine and thereby facilitated the transepithelial taurine transport, which could be inhibited by Pro and Sar, but not BCH. Furthermore, Sar and Pro, but not BCH, decreased taurine C max. The pharmacokinetic investigations showed that intestinal taurine absorption was not saturable at the investigated doses, but that the time ( t max) to reach the maximal plasma concentration ( C max) increased with dose. In vitro studies investigating the transport mechanism of taurine were conducted in human intestinal Caco‐2 cells. BCH is not an inhibitor of PAT1 or the taurine transporter, TauT, hence it was included as a negative control. Oral absorption was measured in the presence of substrates for the proton‐coupled amino acid transporter, PAT1, that is, 200 mg/kg proline (Pro) and sarcosine (Sar), and in the presence of 2‐Amino‐2‐norbornanecarboxylic acid ( BCH) (200 mg/kg). Taurine absorption was measured in male Sprague‐Dawley rats at 10–997 mg/kg and 1–30 mg/kg for oral and intravenous administration, respectively. The aim was to investigate the mechanism for intestinal absorption of taurine in vivo using also in vitro mechanistic studies. Taurine is involved in various physiological processes, and one of the most abundant amino acids in human.
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